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KMID : 0545119960060050326
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Journal of Microbiology and Biotechnology
1996 Volume.6 No. 5 p.326 ~ p.330
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Cloning of Hemolytic Mosquitocidal Delta-endotoxin Gene (cyt) of Bacillus thuringiensis 73E10-2 (serotype 10) into Bacillus subtilis and Characterization of the cyt Gene Product
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KIM, KWANG-HYEON
OHBA, MICHIO/KIM, BYUNG-WOO
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Abstract
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To illustrate whether a hemolysin in δ-endotoxins of Bacillus thuringiensis strain 73E10-2 and subsp. israelensis had immunological identity, a cyt gene of the strain 73E10-2 which encodes a hemolysin was cloned to B. subtilis (transformant 2753). The transformant 2753 containing cyt gene produced the hemolysin which lysed sheep erythocytes after treatment of proteinase K. The hemolysin was proved also to be toxin against mosquito larvae (Aedes aegypti). The milecular weight of the hemolysin produced from the transformant 2753 was determined to be about 25 kDa by SDS-PAGE and immunoblot. The hemolysin in δ-endotoxin of subsp. israelensis and subsp. kyushensis did not react on immunoblot using polyclonal anti- δ-endotoxin of the strain 73E10-2, but 70-140 kDa mosquitocidal toxin in δ-endotoxins of subsp. kyushensis reacted.
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